Isolation of Apoplast

Sammanfattning

The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 mu g of apoplastic proteins can be obtained in a volume of 300 mu L from five potato leaflets, with minimal contamination by non-apoplastic proteins.

Nyckelord

Apoplast; Apoplastic protein; Secretome; Soluble fraction; Protease inhibitor; Phosphate buffer; Vacuum infiltration; Extracellular space

Publicerad i

Methods in Molecular Biology
2017, volym: 1511, nummer: 1511, sidor: 233-240
Titel: Isolation of Plant Organelles and Structures
Utgivare: Springer

SLU författare

• Andreasson, Erik

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

• Abreha, Kibrom

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

• Resjö, Svante

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Molekylärbiologi
Biokemi

Publikationens identifierare

• DOI: https://doi.org/10.1007/978-1-4939-6533-5_18
• ISBN: 978-1-4939-6531-1

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/77554