Kolseth, Anna-Karin
- Institutionen för växtproduktionsekologi, Sveriges lantbruksuniversitet
Sammanfattning
Fusarium langsethiae is a widespread pathogen of small grain cereals, causing problems with T-2 and HT-2 toxin contamination in grains every year. In an effort to better understand the biology of this fungus, we present a draft genome sequence of F. langsethiae Fl201059 isolated from oats in Norway. The assembly was fragmented, but reveals a genome of approximately 37.5 Mb, with a GC content around 48%, and 12,232 predicted protein-coding genes. Focusing on secondary metabolism we identified candidate genes for 12 polyketide synthases, 13 non ribosomal peptide synthetases, and 22 genes for terpene/isoprenoid biosynthesis. Some of these were found to be unique compared to sequence databases. The identified putative Tri5 cluster was highly syntenic to the cluster reported in F. sporotrichioides. Fusarium langsethiae Fl201059 produces a high number of secondary metabolites on Yeast Extract Sucrose (YES) agar medium, dominated by type A trichothecenes. Interestingly we found production of glucosylated HT-2 toxin (Glu-HT-2), previously suggested to be formed by the host plant and not by the fungus itself. In greenhouse inoculations of F. langsethiae FI201059 on barley and oats, we detected the type A trichothecenes: neosolaniol, HT-2 toxin, T-2 toxin, Glu-HT-2 and numerous derivatives of these. (C) 2016 Elsevier B.V. All rights reserved.
Nyckelord
Fusarium langsethiae; Genome; Secondary metabolism; T-2 toxin; HT-2 toxin; Glucosylated mycotoxins; Oats; Cereals
Publicerad i
International Journal of Food Microbiology
2016, volym: 221, sidor: 29-36
Utgivare: ELSEVIER SCIENCE BV
SLU författare
Associerade SLU-program
SLU Nätverk växtskydd
UKÄ forskningsämne
Mikrobiologi
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.ijfoodmicro.2016.01.008
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/82918