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Sammanfattning

Transcription factors (TFs) forming MYB-bHLH-WDR complexes are known to regulate the biosynthesis of specialized metabolites in angiosperms through an intricate network. These specialized metabolites participate in a wide range of biological processes including plant growth, development, reproduction as well as in plant immunity. Studying the regulation of their biosynthesis is thus essential. While MYB (TFs) have been previously shown to control specialized metabolism (SM) in gymnosperms, the identity of their partners, in particular bHLH or WDR members, has not yet been revealed. To gain knowledge about MYB-bHLH-WDR transcription factor complexes in gymnosperms and their regulation of SW, we identified two bHLH homologs of AtTT8, six homologs of the MYB transcription factor 112 2 and one WDR ortholog of Atl I G1 in Norway spruce. We investigated the expression levels of these genes in diverse tissues and upon treatments with various stimuli including methyl-salicylate, methyl-jasmonate, wounding or fungal inoculation. In addition, we also identified protein-protein interactions among different homologs of MYB, bHLH and WDR. Finally, we generated transgenic spruce cell lines overexpressing four of the Norway spruce 112 2 homologs and observed differential regulation of genes in the flavonoid pathway and flavonoid contents.

Nyckelord

MYB; Picea; yeast two-hybrid; TT2; TT8; TTG1; flavonoid

Publicerad i

Frontiers in Plant Science
2017, volym: 8, artikelnummer: 305
Utgivare: FRONTIERS MEDIA SA

SLU författare

Associerade SLU-program

SLU Nätverk växtskydd

UKÄ forskningsämne

Genetik och genomik

Publikationens identifierare

  • DOI: https://doi.org/10.3389/fpls.2017.00305

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/84397