Gel-based phosphoproteomics analysis of sarcoplasmic proteins in postmortem porcine muscle with pH decline rate and time differences

Huang, Honggang; Larsen, Martin R.; Karlsson, Anders H.; Pomponio, Luigi; Costa, Leonardo Nanni; Lametsch, Rene

doi:10.1002/pmic.201100173

Sammanfattning

Meat quality development is highly influenced by the pH decline caused by the postmortem (PM) glycolysis. Protein phosphorylation is an important mechanism in regulating the activity of glycometabolic enzymes. Here, a gel-based phosphoproteomic study was performed to analyze the protein phosphorylation in sarcoplasmic proteins from three groups of pigs with different pH decline rates from PM 1 to 24 h. Globally, the fast pH decline group had the highest phosphorylation level at PM 1 h, but lowest at 24 h, whereas the slow pH decline group showed the reverse case. The same pattern was also observed in most individual bands in 1-DE. The protein phosphorylation levels of 12 bands were significantly affected by the synergy effects of pH and time (p

Nyckelord

Animal proteomics; Association study; Glycolysis; Meat quality; Protein phosphorylation; Porcine sarcoplasmic proteins

Publicerad i

Proteomics
2011, volym: 11, nummer: 20, sidor: 4063-4076
Utgivare: WILEY-BLACKWELL

SLU författare

Karlsson, Anders
- Københavns Universitet

UKÄ forskningsämne

Livsmedelsvetenskap

Publikationens identifierare

DOI: https://doi.org/10.1002/pmic.201100173

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/85665