Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality

Sammanfattning

This study aimed to detect variability in CAST, CAPN1 and CAPN3 porcine genes and to investigate the effect of CAST and CAPN1 polymorphisms on the activity of native and autolyzed mu-calpain and m-calpain, measured from 1 to 72 h post-mortem in Longissimus dorsi (LD) muscle of 30 pigs. Effects of polymorphisms on meat quality parameter such as pH, color and drip loss were also evaluated. Samples carrying CAST EU137105: g.76,872AA genotype showed higher autolyzed mu-calpain activity 24 and 72 h post-mortem, as well as lower drip loss values. Expression of CAST, CAPN1 and CAPN3 was assessed in LD muscles divergent for shear force. Higher CAST and CAPN3 expression was found in LD with high shear force (P

Nyckelord

Calpain activity; CAST; Pork quality; Polymorphism; Proteolysis

Publicerad i

Meat Science
2011, volym: 88, nummer: 4, sidor: 694-700
Utgivare: ELSEVIER SCI LTD

SLU författare

• Karlsson, Anders

  • Københavns Universitet

UKÄ forskningsämne

Livsmedelsvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.meatsci.2011.02.031

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/85670