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Sammanfattning

We have compared the influence of two aromatic cytokinin derivatives, N (6)-benzyladenine (BA) and meta-topolin (mT), on the in vitro multiplication and senescence of wych elm (Ulmus glabra Huds.). After 3 months of cultivation, the micropropagation rate was higher (approx. six times more shoots developed) on Murashige and Skoog (MS) medium supplemented with mT than on MS supplemented with BA. Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT. Differences in efficiency of photosystem II was also detected.

Nyckelord

benzylaminopurine; chlorophyll fluorescence; meta-topolin; organogenesis; rooting; senescence; Ulmus glabra

Publicerad i

Biologia Plantarum
2013, volym: 57, nummer: 1, sidor: 174-178
Utgivare: SPRINGER

SLU författare

  • Karady, Michal

    • Univerzity Palackého v Olomouci

UKÄ forskningsämne

Botanik

Publikationens identifierare

  • DOI: https://doi.org/10.1007/s10535-012-0252-6

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/87410