Walker, William
- United States Department of Agriculture (USDA)
Sammanfattning
Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris.
Nyckelord
extraoral digestion; gene expression; gene knockdown; RNAi; salivary enzyme
Publicerad i
Journal of Insect Science
2010, volym: 10, nummer: 1, artikelnummer: 173
Utgivare: UNIV ARIZONA
SLU författare
UKÄ forskningsämne
Biokemi
Molekylärbiologi
Zoologi
Publikationens identifierare
- DOI: https://doi.org/10.1673/031.010.14133
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/88036