Expression and RNA interference of salivary polygalacturonase genes in the tarnished plant bug, Lygus lineolaris

Sammanfattning

Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris.

Nyckelord

extraoral digestion; gene expression; gene knockdown; RNAi; salivary enzyme

Publicerad i

Journal of Insect Science
2010, volym: 10, nummer: 1, artikelnummer: 173
Utgivare: UNIV ARIZONA

SLU författare

• Walker, William

  • United States Department of Agriculture (USDA)

UKÄ forskningsämne

Zoologi
Biokemi och molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1673/031.010.14133

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/88036