Söderbom, Fredrik
- Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
Sammanfattning
We have detected a surprising heterogeneity among human spliceosomal U1 small nuclear RNA ( snRNA). Most interestingly, we have identified three U1 snRNA variants that lack complementarity to the canonical 59 splice site (5'SS) GU dinucleotide. Furthermore, we have observed heterogeneity among the identified variant U1 snRNA genes caused by single nucleotide polymorphism ( SNP). The identified snRNAs were ubiquitously expressed in a variety of human tissues representing different stages of development and displayed features of functional spliceosomal snRNAs, i.e., trimethylated cap structures, association with Sm proteins and presence in nuclear RNA-protein complexes. The unanticipated heterogeneity among spliceosomal snRNAs could contribute to the complexity of vertebrates by expanding the coding capacity of their genomes.
Nyckelord
U1 snRNP; mRNA splicing; noncanonical 5 ' splice site; SNP; genome complexity
Publicerad i
RNA
2006, volym: 12, nummer: 9, sidor: 1603-1611
Utgivare: COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
SLU författare
Publikationens identifierare
- DOI: https://doi.org/10.1261/rna.26506
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/8912