Cryo-EM Reveals Promoter DNA Binding and Conformational Flexibility of the General Transcription Factor TFIID

Sammanfattning

The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.

Publicerad i

Structure
2009, volym: 17, nummer: 11, sidor: 1442-1452
Utgivare: CELL PRESS

SLU författare

• Linder, Tomas

  • Karolinska institutet

UKÄ forskningsämne

Strukturbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.str.2009.09.007

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/90849