Mass spectrometry captures structural intermediates in protein fiber self-assembly

Abstract

Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.

Published in

Chemical Communications
2017, volume: 53, number: 23, pages: 3319-3322
Publisher: ROYAL SOC CHEMISTRY

SLU Authors

• Andersson, Marlene

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Johansson, Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Karolinska Institute

• Rising, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Molecular Biology
Biochemistry

Publication identifier

• DOI: https://doi.org/10.1039/c7cc00307b

Permanent link to this page (URI)

https://res.slu.se/id/publ/90938