Mass spectrometry captures structural intermediates in protein fiber self-assembly

Sammanfattning

Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.

Publicerad i

Chemical Communications
2017, volym: 53, nummer: 23, sidor: 3319-3322
Utgivare: ROYAL SOC CHEMISTRY

SLU författare

• Andersson, Marlene

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Johansson, Jan

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    
  • Karolinska institutet

• Rising, Anna

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Molekylärbiologi
Biokemi

Publikationens identifierare

• DOI: https://doi.org/10.1039/c7cc00307b

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/90938