Tocopherol levels in different mango varieties correlate with MiHPPD expression and its over-expression elevates tocopherols in transgenic Arabidopsis and tomato

Sammanfattning

Mango fruit tocopherol levels vary in different varieties during ripening. This study shows that tocopherol accumulation is highly correlated with its p-hydroxyphenyl pyruvate dioxygenase (MiHPPD) gene expression during ripening. MiHPPD transcript is ethylene induced and differentially expressed in four mango varieties used in this study. Higher/lower accumulation of tocopherol (mainly alpha-tocopherol) was achieved by heterologous expression of MiHPPD in Arabidopsis and tomato. The results suggest that tocopherol accumulation in mango fruit is correlated to MiHPPD gene expression. Over-expression of MiHPPD gene channelizes the flux towards tocophreol biosynthesis and could be used as a potential tool for metabolic engineering.

Nyckelord

Arabidopsis; HPPD; Mango; Tocopherol; Tomato; Transgenic

Publicerad i

3 BIOTECH
2017, volym: 7, artikelnummer: 352
Utgivare: SPRINGER HEIDELBERG

SLU författare

• Singh, Rajesh

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    
  • Council of Scientific and Industrial Research (CSIR)

UKÄ forskningsämne

Växtbioteknologi

Publikationens identifierare

• DOI: https://doi.org/10.1007/s13205-017-0991-3

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/92960