Lafon-Placette, Clément
- Institut national de la recherche agronomique (INRA)
Sammanfattning
The adaptive capacity of long-lived organisms such as trees to the predicted climate changes, including severe and successive drought episodes, will depend on the presence of genetic diversity and phenotypic plasticity. Here, the involvement of epigenetic mechanisms in phenotypic plasticity toward soil water availability was examined in Populusxeuramericana. This work aimed at characterizing (i) the transcriptome plasticity, (ii) the genome-wide plasticity of DNA methylation, and (iii) the function of genes affected by a drought-rewatering cycle in the shoot apical meristem. Using microarray chips, differentially expressed genes (DEGs) and differentially methylated regions (DMRs) were identified for each water regime. The rewatering condition was associated with the highest variations of both gene expression and DNA methylation. Changes in methylation were observed particularly in the body of expressed genes and to a lesser extent in transposable elements. Together, DEGs and DMRs were significantly enriched in genes related to phytohormone metabolism or signaling pathways. Altogether, shoot apical meristem responses to changes in water availability involved coordinated variations in DNA methylation, as well as in gene expression, with a specific targeting of genes involved in hormone pathways, a factor that may enable phenotypic plasticity.
Nyckelord
Differentially expressed genes; differentially methylated regions; DNA methylation; phenotypic plasticity; Populusxeuramericana; shoot apical meristem; water availability
Publicerad i
Journal of Experimental Botany
2018, volym: 69, nummer: 3, sidor: 537-551
Utgivare: OXFORD UNIV PRESS
SLU författare
UKÄ forskningsämne
Genetik och genomik
Botanik
Publikationens identifierare
- DOI: https://doi.org/10.1093/jxb/erx409
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/94313