Lage, Sandra
- Institutionen för vilt, fisk och miljö, Sveriges lantbruksuniversitet
Sammanfattning
A systematic qualitative and quantitative analysis of fatty acid methyl esters (FAMEs) is crucial for microalgae species selection for biodiesel production. The aim of this study is to identify the best method to assess microalgae FAMEs composition and content. A single-step method, was tested with and without purification steps-that is, separation of lipid classes by thin-layer chromatography (TLC) or solid-phase extraction (SPE). The efficiency of a direct transesterification method was also evaluated. Additionally, the yield of the FAMEs and the profiles of the microalgae samples with different pretreatments (boiled in isopropanol, freezing, oven-dried and freeze-dried) were compared. The application of a purification step after lipid extraction proved to be essential for an accurate FAMEs characterisation. The purification methods, which included TLC and SPE, provided superior results compared to not purifying the samples. Freeze-dried microalgae produced the lowest FAMEs yield. However, FAMEs profiles were generally equivalent among the pretreatments.
Nyckelord
Direct transesterification; Lipids; Solid-phase extraction; Thin-layer chromatography; Wastewater
Publicerad i
Bioresource Technology
2018, volym: 257, sidor: 121-128
Utgivare: ELSEVIER SCI LTD
SLU författare
Gentili, Francesco
- Institutionen för vilt, fisk och miljö, Sveriges lantbruksuniversitet
- Institutionen för vilt, fisk och miljö, Sveriges lantbruksuniversitet
Globala målen (SDG)
UKÄ forskningsämne
Bioenergi
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.biortech.2018.01.153
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/95027