Jiwakanon, Jatesada
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
The aim of this study was to investigate the influence of pre- and post-ovulatory insemination and early pregnancy on the distribution of immune cells in the oviduct. Eighteen sows were pre-ovulatory and sixteen sows were post-ovulatory inseminated and slaughtered at different times, 5-6 h after insemination, 20-25 h and approximately 70 h after ovulation, day 11 and day 19. Immediately after slaughter, oviductal samples of three different segments (isthmus, ampulla and infundibulum) were fixed, embedded in plastic resin and stained with toluidine blue or cryofixed and stored in a freezer at -70 degrees C until analysed by immunohistochemistry (pre-ovulatory inseminated sows) with an avidin-biotin peroxidase method. Quantitative and qualitative examinations of oviductal epithelium and subepithelial connective tissue were performed by light microscopy. After pre- or post-ovulatory insemination, neutrophils were not observed in the oviductal epithelium from any of the segments or groups. The numbers of intraepithelial lymphocytes of all sows as well as CD2- and CD3-positive cells of the pre-ovulatory inseminated sows were higher in the infundibulum than in the other segments (p
Publicerad i
Reproduction in Domestic Animals
2006, volym: 41, nummer: 5, sidor: 455-466
Utgivare: BLACKWELL PUBLISHING
SLU författare
Persson, Elisabeth
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Dalin, Anne-Marie
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Husdjursvetenskap
Veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1111/j.1439-0531.2006.00695.x
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/9761