Backbone nuclear magnetic resonance assignment of human deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)

Sammanfattning

Nuclear-associated deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) is an enzyme that hydrolyses deoxyuridine 5'-triphosphate (dUTP) to the monophosphate, thereby controlling the dUTP levels of the organism, which is essential for survival. Further, dUTPase is up-regulated in many cancers. Thus, dUTPase is a highly interesting potential drug target. We report, for the first time, the near complete nuclear magnetic resonance (NMR) spectroscopy N-15/C-13/H-1 backbone assignment of the 3 x 164 amino acids homo-trimer human dUTPase. Previously, only a handful backbone resonances belonging to the flexible C-terminus has been published for any protein in the dUTPase family.

Nyckelord

Human dUTPase; Protein backbone assignment; Multidimensional NMR

Publicerad i

Biomolecular NMR Assignments
2014, volym: 8, nummer: 1, sidor: 81-84
Utgivare: SPRINGER

SLU författare

• Agback, Tatiana

  • Medivir AB

UKÄ forskningsämne

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Publikationens identifierare

• DOI: https://doi.org/10.1007/s12104-013-9457-7

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/99458