Björkman, Camilla
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
On two occasions, we identified Cryptosporidium parvum with an unusual banding pattern after restriction enzyme digestion of Cryptosporidium oocyst wan protein PCR fragments. The samples originated from a single farm with calf diarrhoea. The Cryptosporidium oocyst wall protein PCR fragment sequence revealed a point mutation in one of the recognition sites for RsaI. Our subgenotyping showed that samples from both samplings shared the same MS1 and GP15 alleles. Analysis of the TP14 microsatellite was less sensitive, and results were only obtained from two samples. Both had the same allele. This is, to our knowledge, the first description of a C. parvum with this Cryptosporidium oocyst wall protein genotype. More importantly, the genotype seems to be stable over time since we could detect it again among newborn animals almost 1 year after it was first identified.
Nyckelord
Cryptosporidium parvum; bovine infection; Cryptosporidium oocyst wall protein (COWP); 18S rRNA; microsatellites; persistence
Publicerad i
FEMS Microbiology Letters
2006, volym: 254, nummer: 1, sidor: 71-74
Utgivare: BLACKWELL PUBLISHING
SLU författare
Mattsson, Jens
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Husdjursvetenskap
Veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1111/j.1574-6968.2005.00021.x
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/9958