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Forskningsartikel - Refereegranskat, 2020

Nursing and training of pigs used in renal transplantation studies

Ryden, Anneli; Manell, Elin; Biglarnia, Alireza; Hedenqvist, Patricia; Strandberg, Gabriel; Ley, Charles; Hansson, Kerstin; Nyman, Gorel; Jensen-Waern, Marianne

Sammanfattning

The pig is commonly used in renal transplantation studies since the porcine kidney resembles the human kidney. To meet the requirements of intense caretaking and examination without stress, a 2-week socialisation and training programme was developed. Conventional cross-breed pigs (n = 36) with high health status were trained for 15 min/day in a four-step training programme before kidney transplantation. The systematic training resulted in calm animals, which allowed for ultrasound examination, blood sampling and urine sampling without restraint. When a 2-methacryloyloxyethyl phosphorylcholine polymer-coated jugular catheter introduced via the auricular vein was used for post-operative blood sampling, clotting was avoided. To assess renal function, urinary output was observed and creatinine and cystatin C were measured; the latter was not found to be useful in recently transplanted pigs. The results presented contribute to the 3Rs (refine, reduce, replace).

Nyckelord

swine; urinary bladder; indwelling catheter; kidney transplantation

Publicerad i

Laboratory Animals
2020, Volym: 54, nummer: 5, artikelnummer: 0023677219879169
Utgivare: SAGE PUBLICATIONS INC