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Research article2002Peer reviewed

The DNA vaccine vector pcDNA3 induces IFN-alpha production in pigs

Johansson, E; Wallgren, P; Fuxler, L; Domeika, K; Lefevre, F; Fossum, C

Abstract

The cytokine inducing capacity of the vaccine vector pcDNA3, a methylated form of the plasmid, and pcDNA3 encoding porcine interleukin (IL)-6 or granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in pigs, using a model with tissue chambers implanted subcutaneously. The production of interferon (IFN)-alpha, IFN-gamma, IL-6 and GM-CSF was studied at local (tissue chamber fluid (TCF)) and systemic (serum) levels during 3 days post-injection. All forms of the plasmid, except the methylated, induced a transient local production of IFN-alpha but no plasmid-induced production of IFN-gamma, GM-CSF or IL-6 could be detected after injection of the plasmids. The IFN-alpha response increased markedly at repeated injections of pcDNA3. This IFN-alpha inducing capacity of the plasmid is likely to affect immune responses at DNA vaccination of pigs. (C) 2002 Elsevier Science B.V. All rights reserved.

Keywords

pig; plasmid; immunostimulatory DNA; cytokines

Published in

Veterinary Immunology and Immunopathology
2002, Volume: 87, number: 1-2, article number: PII S0165-2427(02)00027-7Publisher: ELSEVIER SCIENCE BV

      SLU Authors

    • Fossum, Caroline

      • Department of Molecular Biosciences, Swedish University of Agricultural Sciences

      • Fuxler, Lisbeth

        • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences

        • Domeika, Kristina

          • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Pathobiology

        Publication identifier

        DOI: https://doi.org/10.1016/S0165-2427(02)00024-7

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/106619