Abstract

The cytokine inducing capacity of the vaccine vector pcDNA3, a methylated form of the plasmid, and pcDNA3 encoding porcine interleukin (IL)-6 or granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in pigs, using a model with tissue chambers implanted subcutaneously. The production of interferon (IFN)-alpha, IFN-gamma, IL-6 and GM-CSF was studied at local (tissue chamber fluid (TCF)) and systemic (serum) levels during 3 days post-injection. All forms of the plasmid, except the methylated, induced a transient local production of IFN-alpha but no plasmid-induced production of IFN-gamma, GM-CSF or IL-6 could be detected after injection of the plasmids. The IFN-alpha response increased markedly at repeated injections of pcDNA3. This IFN-alpha inducing capacity of the plasmid is likely to affect immune responses at DNA vaccination of pigs. (C) 2002 Elsevier Science B.V. All rights reserved.

Keywords

pig; plasmid; immunostimulatory DNA; cytokines

Published in

Veterinary Immunology and Immunopathology
2002, Volume: 87, number: 1-2, article number: PII S0165-2427(02)00027-7
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Fossum, Caroline

  • Department of Molecular Biosciences, Swedish University of Agricultural Sciences
    

• Fuxler, Lisbeth

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

• Domeika, Kristina

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Pathobiology


Publication identifier

DOI: https://doi.org/10.1016/S0165-2427(02)00024-7

Permanent link to this page (URI)

https://res.slu.se/id/publ/106619