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Forskningsartikel2021Vetenskapligt granskad

Dual fluorescent immunochromatographic assay for simultaneous quantitative detection of citrinin and zearalenone in corn samples

Xu, Ying; Ma, Biao; Chen, Erjing; Yu, Xiaoping; Ye, Zihong; Sun, Chuanxin; Zhang, Mingzhou

Sammanfattning

The presence of multiple mycotoxins in the agricultural products poses a serious threat to the health of humans and animals. Citrinin (CIT) causes slow growth in animals and damages the kidney function. Zearalenone (ZEN) causes chronic poisoning, abnormal functioning and even death in animals. Herein, a dual fluorescent immunochromatographic assay (DF-ICA) based on europium nanoparticles (EuNPs) was developed for the simultaneous detection of CIT and ZEN in the corn samples. After optimization, the limits of detection (LODs), IC50 and average recoveries for the simultaneous determination of CIT and ZEN were 0.06 and 0.11 ng/mL, 0.35 and 0.76 ng/mL, from 86.3% to 111.6% and from 86.6% to 114.4%, respectively. Moreover, the DF-ICA was validated by high performance liquid chromatography (HPLC) analyses, and a satisfactory consistency was obtained. In brief, this work demonstrates the feasibility of DF-ICA for simultaneous monitoring of CIT and ZEN in the corn samples.

Nyckelord

Fluorescent immunochromatographic assay; Europium nanoparticles; Simultaneous detection; Citrinin; Zearalenone; Corn

Publicerad i

Food Chemistry
2021, Volym: 336, artikelnummer: 127713Utgivare: ELSEVIER SCI LTD

    UKÄ forskningsämne

    Livsmedelsvetenskap

    Publikationens identifierare

    DOI: https://doi.org/10.1016/j.foodchem.2020.127713

    Permanent länk till denna sida (URI)

    https://res.slu.se/id/publ/108249