Sun, Chuanxin
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Forskningsartikel2020Vetenskapligt granskadÖppen tillgång
Xu, Ying; Ma, Biao; Chen, Erjing; Yu, Xiaoping; Sun, Chuanxin; Zhang, Mingzhou
An ultrahigh-sensitivity lateral flow immunochromatography (LFIC) assay based on up-converting nanoparticles (UCNPs) was developed to carry out a multi-residue detection of tetracycline in milk. The sensitivity of the immunoassay was greatly improved by the use of a broad-spectrum monoclonal antibody attached to UCNPs to form a signal probe. Under the optimal conditions, the UCNP-LFIC assay enabled sensitive detection of tetracycline (TC) as well as of oxytetracycline (OTC), chlortetracycline (CTC), and doxycycline (DOX) within 10 min, withIC(50)values of 0.32, 0.32, 0.26, 0.22 ng/mL, respectively. There was no cross-reactivity with ten other antibiotics. Similarly, we evaluated the experimental results for matrix effects. Experiments involving spiking showed the four tetracycline antibiotics displaying mean recoveries ranging from 93.95 to 111.90% with relative standard deviations (RSDs) of < 9.95%. The detection results of actual samples using the developed method showed a good correlation (R-2 >= 0.98) with the results using high-performance liquid chromatography (HPLC). Thus, the assay can achieve an ultrahighly sensitive detection of antibiotics in milk, and can hence promote human health and provides promising applications in the bio-detection field.
up-conversion nanoparticle; tetracycline; immunoassay; multi-residue detection; milk
Frontiers in Chemistry
2020, Volym: 8, artikelnummer: 759Utgivare: FRONTIERS MEDIA SA
Analytisk kemi
DOI: https://doi.org/10.3389/fchem.2020.00759
https://res.slu.se/id/publ/108629