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Research article - Peer-reviewed, 2020

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

Johannisson, Anders; Al-Essawe, Essraa M; Al-Saffar, Anas Kh.; Karkehabadi, Saeid; Lima Verde, Isabel; Wulf, Manuela; Aurich, Christine; Morrell, Jane;

Abstract

The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparinbinding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of nonheparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season.

Keywords

chromatography; cytometry; protein fractions; Seasonal changes; stallion

Published in

Journal of Reproduction and Development

2020, volume: 66, number: 3, pages: 215-221

Authors' information

Swedish University of Agricultural Sciences, Department of Clinical Sciences
Al-Essawe, Essraa M
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Al-Essawe, Essraa M
Al-Nahrain University
Al-Saffar, Anas Kh.
Uppsala University
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Lima Verde, Isabel (Lima Verde, Isabel)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Wulf, Manuela
Graf Lehndorff Institute for Equine Science
Aurich, Christine
University of Veterinary Medicine Vienna (Vetmeduni)
Swedish University of Agricultural Sciences, Department of Clinical Sciences

UKÄ Subject classification

Clinical Science

Publication Identifiers

DOI: https://doi.org/10.1262/jrd.2019-072

URI (permanent link to this page)

https://res.slu.se/id/publ/108786