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Research article - Peer-reviewed, 2020

Mouse Strain-Dependent Difference Toward the Staphylococcus aureus Allergen Serine Protease-Like Protein D Reveals a Novel Regulator of IL-33

Teufelberger, Andrea R.; Van Nevel, Sharon; Hulpiau, Paco; Nordengrun, Maria; Savvides, Savvas N.; De Graeve, Sarah; Akula, Srinivas; Holtappels, Gabriele; De Ruyck, Natalie; Declercq, Wim; Vandenabeele, Peter; Hellman, Lars; Broker, Barbara M.; Krysko, Dmitri, V; Bachert, Claus; Krysko, Olga


Staphylococcus aureus(S. aureus)can secrete a broad range of virulence factors, among which staphylococcal serine protease-like proteins (Spls) have been identified as bacterial allergens. TheS. aureusallergen serine protease-like protein D (SplD) induces allergic asthma in C57BL/6J mice through the IL-33/ST2 signaling axis. Analysis of C57BL/6J, C57BL/6N, CBA, DBA/2, and BALB/c mice treated with intratracheal applications of SplD allowed us to identify a frameshift mutation in the serine (or cysteine) peptidase inhibitor, clade A, and member 3I (Serpina3i) causing a truncated form of SERPINA3I in BALB/c, CBA, and DBA/2 mice. IL-33 is a key mediator of SplD-induced immunity and can be processed by proteases leading to its activation or degradation. Full-length SERPINA3I inhibits IL-33 degradationin vivoin the lungs of SplD-treated BALB/c mice andin vitroby direct inhibition of mMCP-4. Collectively, our results establish SERPINA3I as a regulator of IL-33 in the lungs following exposure to the bacterial allergen SplD, and that the asthma phenotypes of mouse strains may be strongly influenced by the observed frameshift mutation in Serpina3i. The analysis of this protease-serpin interaction network might help to identify predictive biomarkers for type-2 biased airway disease in individuals colonized byS. aureus.


allergy; asthma; IL-33; S; aureus; SplD; type 2 immunity

Published in

Frontiers in Immunology
2020, volume: 11, article number: 582044

Authors' information

Teufelberger, Andrea R.
Ghent University
Van Nevel, Sharon
Ghent University
Hulpiau, Paco
Ghent University
Nordengrün, Maria
University of Greifswald
Savvides, Savvas N.
Ghent University
De Graeve, Sarah
Ghent University
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Holtappels, Gabriele
Ghent University
De Ruyck, Natalie
Ghent University
Declercq, Wim
Ghent University
Vandenabeele, Peter
Ghent University
Hellman, Lars
Uppsala University
Bröker, Barbara M.
University of Greifswald
Krysko, Dmitri
Lobachevsky State University of Nizhni Novgorod
Bachert, Claus
Karolinska University Hospital
Krysko, Olga
Ghent University

UKÄ Subject classification

Biochemistry and Molecular Biology

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