Skip to main content
SLU publication database
Research article - Peer-reviewed, 2021

Comparative genomics with a multidrug-resistant Klebsiella pneumoniae isolate reveals the panorama of unexplored diversity in Northeast Brazil

Profeta, Rodrigo; Seyffert, Nubia; Tiwari, Sandeep; Viana, Marcus V. C.; Jaiswal, Arun Kumar; Caetano, Ana Carolina; Bucker, Daniel Henrique; de Oliveira, Luciana Tavares; Santos, Roselane; Gala-Garcia, Alfonso; Kato, Rodrigo B.; Padilha, Francine F.; Lima-Verde, Isabel B.; Ghosh, Preetam; Barh, Debmalya; Goes-Neto, Aristoteles; Figueiredo, Henrique C. P.; Soares, Siomar C.; Meyer, Roberto; Brenig, Bertram;
Show more authors

Abstract

The emergence of community acquired infections increases the public health concern on K. pneumoniae and closely related bacteria among which antimicrobial resistance spreads. We report a multidrug-resistant K. pneumoniae isolate, B31, of a patient infected in the community and admitted to an intensive care unit in Northeast Brazil. Antimicrobial susceptibility and genome information were thoroughly investigated to characterize B31 in front of 172 sequenced strains of different countries. Assigned to the Sequence Type 15, which is globally spread, B31 presented extended spectrum beta-lactamase, tigecycline and ciprofloxacin resistance. Genome sequencing revealed most resistance genes being carried by plasmids with high dissemination potential. The absence of main virulence factors, like yersiniabactin and colibactin, apparently suggests a mild pathogenic strain which, on the contrary, persisted and caused severe infection in a previously healthy patient. The present study contributes to unveil the unclear genomic scenario of virulent and multidrug-resistant K. pneumoniae in Brazil.

Keywords

Klebsiella pneumoniae; Multilocus Sequence Type; Hospital Emergency Care; Phylogenomics; Antimicrobial Evaluation; Virulence Genes

Published in

Gene
2021, volume: 772, article number: 145386
Publisher: ELSEVIER

Authors' information

Profeta, Rodrigo
Universidade Federal de Minas Gerais
Seyffert, Nubia
Universidade Federal de Minas Gerais
Tiwari, Sandeep
Universidade Federal de Minas Gerais
Viana, Marcus V. C.
Universidade Federal de Minas Gerais
Jaiswal, Arun Kumar
Universidade Federal de Minas Gerais
Caetano, Ana Carolina
Universidade Federal de Minas Gerais
Bucker, Daniel Henrique
Universidade Federal de Minas Gerais
de Oliveira, Luciana Tavares
Universidade Federal de Minas Gerais
Santos, Roselane
Universidade Federal de Minas Gerais
Gala-Garcia, Alfonso
Universidade Federal de Minas Gerais
Kato, Rodrigo B.
Universidade Federal de Minas Gerais
Padilha, Francine F.
Universidade Tiradentes
Lima Verde, Isabel (Lima Verde, Isabel)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Ghosh, Preetam
Virginia Commonwealth University
Barh, Debmalya
Institute of Integrative Omics and Applied Biotechnology (IIOAB)
Goes-Neto, Aristoteles
Universidade Federal de Minas Gerais
Figueiredo, Henrique C. P.
Universidade Federal de Minas Gerais
Soares, Siomar C.
Universidade Federal de Minas Gerais
Meyer, Roberto
Universidade Federal da Bahia
Brenig, Bertram
University of Gottingen
Show more authors

Sustainable Development Goals

SDG3 Good health and well-being

UKÄ Subject classification

Microbiology in the medical area
Medical Genetics

Publication Identifiers

DOI: https://doi.org/10.1016/j.gene.2020.145386

URI (permanent link to this page)

https://res.slu.se/id/publ/111098