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Research article - Peer-reviewed, 2021

Chicken-eaters and pork-eaters have different gut microbiota and tryptophan metabolites

Shi, Jie; Zhao, Di; Zhao, Fan; Wang, Chong; Zamaratskaia, Galia; Li, Chunbao


This study was aimed to evaluate the differences in the composition of gut microbiota, tryptophan metabolites and short-chain fatty acids in feces between volunteers who frequently ate chicken and who frequently ate pork. Twenty male chicken-eaters and 20 male pork-eaters of 18 and 30 years old were recruited to collect feces samples for analyses of gut microbiota composition, short-chain fatty acids and tryptophan metabolites. Chicken-eaters had more diverse gut microbiota and higher abundance of Prevotella 9, Dialister, Faecalibacterium, Megamonas, and Prevotella 2. However, pork-eaters had higher relative abundance of Bacteroides, Faecalibacterium, Roseburia, Dialister, and Ruminococcus 2. In addition, chicken-eaters had high contents of skatole and indole in feces than pork-eaters, as well as higher contents of total short chain fatty acids, in particular for acetic acid, propionic acid, and branched chain fatty acids. The Spearman's correlation analysis revealed that the abundance of Prevotella 2 and Prevotella 9 was positively correlated with levels of fecal skatole, indole and short-chain fatty acids. Thus, intake of chicken diet may increase the risk of skatole- and indole-induced diseases by altering gut microbiota.

Published in

Scientific Reports
2021, volume: 11, number: 1, article number: 11934

Authors' information

Shi, Jie
Nanjing Agricultural University
Zhao, Di
Nanjing Agricultural University
Zhao, Fan
Nanjing Agricultural University
Wang, Chong
Nanjing Agricultural University
Swedish University of Agricultural Sciences, Department of Molecular Sciences
University of South Bohemia in České Budějovice
Li, Chunbao
Nanjing Agricultural University

UKÄ Subject classification

Nutrition and Dietetics

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