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Research article - Peer-reviewed, 2021

Comparison of Quality Changes in Eurasian Perch (Perca fluviatilis L.) Fillets Originated from Two Different Rearing Systems during Frozen and Refrigerated Storage

Hematyar, Nima; Mraz, Jan; Stejskal, Vlastimil; Sampels, Sabine; Linhartova, Zuzana; Prokesova, Marketa; Vacha, Frantisek; Krizek, Martin; Dadakova, Eva; Sondergard Moller, Hanne; Kastrup Dalsgaard, Trine


The current knowledge on how different Eurasian perch rearing systems impact the final fillet quality is scant. Therefore, two domestic storage conditions were investigated-10 months frozen (-20 degrees C) and 12 days refrigerated (+4 degrees C) storage conditions-in order to determine (i) how the choice of rearing system affects fillets quality during different processing conditions and (ii) if oxidative changes and other quality parameters were interactive. For the proposed idea, proteome analysis, oxidative changes, and some quality parameters were considered in this study. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) indicated a higher loss of protein in the frozen fillets from ponds (PF) than the fillets from recirculating aquaculture systems (RAS) (RF). Western blot showed a higher protein carbonyls level in RF compared to PF, which was confirmed by the total protein carbonyls during frozen storage. PF indicated less liquid loss, hardness, and oxidation progress than RF in both storage conditions. The biogenic amines index (BAI) in the fillets from either origin showed acceptable levels during storage at +4 degrees C. Furthermore, the n-3/n-6 ratio was similar for both fillets. The deterioration of fillets during frozen storage was mainly caused by formation of ice crystals followed by protein oxidation, while protein oxidation was the main concern during refrigerated storage confirmed by principal component analysis (PCA) analysis.


rearing systems; fillet quality; protein characterization; hardness; liquid loss

Published in

2021, volume: 10, number: 6, article number: 1405
Publisher: MDPI

Authors' information

Hematyar, Nima
University of South Bohemia Ceske Budejovice
Mraz, Jan
University of South Bohemia Ceske Budejovice
Stejskal, Vlastimil
University of South Bohemia Ceske Budejovice
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Linhartova, Zuzana
University of South Bohemia Ceske Budejovice
Prokesova, Marketa
University of South Bohemia Ceske Budejovice
Vacha, Frantisek
University of South Bohemia Ceske Budejovice
Krizek, Martin
University of South Bohemia Ceske Budejovice
Dadakova, Eva
University of South Bohemia Ceske Budejovice
Sondergard Moller, Hanne
Aarhus University
Kastrup Dalsgaard, Trine
Aarhus University

UKÄ Subject classification

Food Science

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