Elander, Pernilla
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Forskningsartikel2021Vetenskapligt granskadÖppen tillgång
Gutierrez-Beltran, Emilio; Elander, Pernilla H.; Dalman, Kerstin; Dayhoff, Guy W.; Moschou, Panagiotis N.; Uversky, Vladimir N.; Crespo, Jose L.; Bozhkov, Peter, V
Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein-protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.
heat stress; intrinsically disordered regions; SnRK1; SNF1; AMPK; stress granules; tudor staphylococcal nuclease
The Embo Journal
2021, Volym: 40, nummer: 17, artikelnummer: e105043Utgivare: WILEY
Biokemi och molekylärbiologi
DOI: https://doi.org/10.15252/embj.2020105043
https://res.slu.se/id/publ/113227