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Research article - Peer-reviewed, 2021

Relationship of motility activation to lipid composition, protein profile, and swelling rate of burbot Lota lota spermatozoon following change of temperature and osmolality

Dadras, Hadiseh; Policar, Tomas; Loginov, Dmitry; Boryshpolets, Sergii; Loginova, Yana; Sampels, Sabine; Sotnikov, Anatolii; Kholodnyy, Vitaliy; Sterba, Jan; Xin, Miaomiao; Dzyuba, Borys

Abstract

Despite available information on the roles of osmotic pressure, potassium (K+), and calcium (Ca2+) in activation of Eurasian burbot spermatozoon motility, the changes in milt biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is still unclear. We explored spermatozoon swelling, lipid composition and proteome in relation to osmolality and temperature of swimming medium. The result revealed that temperature increment from 4 to 30°C increases burbot spermatozoa vulnerability to osmotic pressure, decreasing motility in extremely hypotonic media, and the presence of Ca2+ decreases swelling of spermatozoa. No spermatozoon swelling was observed in non-ionic isotonic media at all studied temperatures. A role of swelling in activation of spermatozoa motility at 30°C was rejected. No differences were found in protein profile and lipid composition with respect to activation of burbot spermatozoa at 30°C. Burbot spermatozoon activation occurs at 30°C without modification of the spermatozoon membrane. Elucidation of the intrinsic signaling pathway of burbot spermatozoon spontaneous activation requires further study.

Keywords

biomolecule; fish; lipid; osmolality; protein; sperm activation

Published in

Frontiers in marine science
2021, volume: 8, article number: 736087

Authors' information

Dadras, Hadiseh
University of South Bohemia in České Budějovice
Policar, Tomas
University of South Bohemia in České Budějovice
Loginov, Dmitry
University of South Bohemia in České Budějovice
Boryshpolets, Sergii
University of South Bohemia in České Budějovice
Loginova, Yana
University of South Bohemia in České Budějovice
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Sotnikov, Anatolii
University of South Bohemia in České Budějovice
Kholodnyy, Vitaliy
University of South Bohemia in České Budějovice
Sterba, Jan
University of South Bohemia in České Budějovice
Xin, Miaomiao
University of South Bohemia in České Budějovice
Dzyuba, Borys
University of South Bohemia in České Budějovice

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.3389/fmars.2021.736087

URI (permanent link to this page)

https://res.slu.se/id/publ/113936