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Forskningsartikel2021Vetenskapligt granskadÖppen tillgång

Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda

Balinandi, Stephen; von Bromssen, Claudia; Tumusiime, Alex; Kyondo, Jackson; Kwon, Hyesoo; Monteil, Vanessa M.; Mirazimi, Ali; Lutwama, Julius; Mugisha, Lawrence; Malmberg, Maja


Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne viral hemorrhagic disease caused by Crimean-Congo Hemorrhagic Fever Virus (CCHFV) that poses serious public health challenges in many parts of Africa, Europe and Asia.Methods: We examined 500 cattle sera samples from five districts for CCHFV antibodies using in-house and commercially available (IDVet) ELISA, Immunofluorescent assay (IFA) and Real-time polymerase chain reaction (RT-PCR).Results: 500 cattle (73.8 % females) were analyzed; CCHFV seropositivity was 12.6 % (n = 63) and 75.0 % (n = 375) with the in-house and IDVet ELISAs, respectively. Seropositivity was associated with geographical location, increasing age, being female, and having a higher tick burden. Twenty four out of the 37 (64.8 %) were seropositive for CCHFV using IFA and all were negative for virus on RT-PCR. The IFA results were more com-parable to IDVet (kappa(coefficient) = 0.88, p = <0.01) than to in-house (kappa(coefficient) = 0.32, p = 0.02).Conclusions: Our study confirmed the presence and high prevalence of anti-CCHF antibodies in cattle based on three methods from all the five study districts, confirming presence and exposure of CCHFV. Given the zoonotic potential for CCHFV, we recommend a multidisciplinary public health surveillance and epidemiology of CCHFV in both animals and humans throughout the country.


Crimean-Congo Hemorrhagic Fever Virus; Seroprevalence; Cattle; Uganda; Immunofluorescent assay

Publicerad i

Journal of Virological Methods
2021, Volym: 290, artikelnummer: 114075
Utgivare: ELSEVIER