Simha, Prithvi
- Institutionen för energi och teknik, Sveriges lantbruksuniversitet
Forskningsartikel2022Vetenskapligt granskadÖppen tillgång
Yadav, Ashish; Simha, Prithvi; Sathe, Priyanka; Gantayet, Lalit Mohan; Pandit, Aniruddha
In this study, we designed, developed, and optimized an enzymatic fiber degumming process. First, a Partially Purified Enzyme Solution (PPES) containing pectinase, cellulase and xylanase was co-produced via ultrasound-assisted fermentation of orange peels using Bacillus subtilis ABDR01. Then, degumming with PPES was optimized for pH, temperature, incubation time and PPES concentration. Finally, three sets of ultrasound-assisted degumming experiments - chemical, enzymatic, and coupled enzymatic-chemical degumming with reduced alkali concentration (0.5% NaOH, 30 min) were performed. Chemical degumming for 2 h with 1% NaOH at 98 degrees C resulted in residual gum content of 5.9%, whereas ultrasound-assisted (30 min, 25 kHz) chemical degumming reduced the gum content to 3.1%. At a pH of 7.5, temperature of 45 degrees C, 30 h incubation and 2% PPES, gum removal efficiency of >60% and fibers with <10% residual gum were produced. Coupling the ultrasound-assisted enzymatic degumming with reduced alkali chemical degumming resulted in gum content of <3%. (C) 2022 The Author(s). Published by Elsevier B.V.
Degumming; Enzyme; Fiber strength; Process optimization; Textile processing; Ultrasonication
Environmental technology & innovation
2022, Volym: 28, artikelnummer: 102666
Utgivare: ELSEVIER
Biokatalys och enzymteknik
DOI: https://doi.org/10.1016/j.eti.2022.102666
https://res.slu.se/id/publ/118223