Skip to main content
Research article - Peer-reviewed, 2022

Quantitative Transcriptome Analysis of Purified Equine Mast Cells Identifies a Dominant Mucosal Mast Cell Population with Possible Inflammatory Functions in Airways of Asthmatic Horses

Akula, Srinivas; Riihimäki, Miia; Waern, Ida; Åbrink, Magnus; Raine, Amanda; Hellman, Lars; Wernersson, Sara

Abstract

Asthma is a chronic inflammatory airway disease and a serious health problem in horses as well as in humans. In humans and mice, mast cells (MCs) are known to be directly involved in asthma pathology and subtypes of MCs accumulate in different lung and airway compartments. The role and phenotype of MCs in equine asthma has not been well documented, although an accumulation of MCs in bronchoalveolar lavage fluid (BALF) is frequently seen. To characterize the phenotype of airway MCs in equine asthma we here developed a protocol, based on MACS Tyto sorting, resulting in the isolation of 92.9% pure MCs from horse BALF. We then used quantitative transcriptome analyses to determine the gene expression profile of the purified MCs compared with total BALF cells. We found that the MCs exhibited a protease profile typical for the classical mucosal MC subtype, as demonstrated by the expression of tryptase (TPSB2) alone, with no expression of chymase (CMA1) or carboxypeptidase A3 (CPA3). Moreover, the expression of genes involved in antigen presentation and complement activation strongly implicates an inflammatory role for these MCs. This study provides a first insight into the phenotype of equine MCs in BALF and their potential role in the airways of asthmatic horses.

Keywords

mast cell; transcriptome; asthma; airway; horse

Published in

International Journal of Molecular Sciences
2022, volume: 23, number: 22, article number: 13976

Authors' information

Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Raine, Amanda
Uppsala University
Hellman, Lars
Uppsala University
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)

UKÄ Subject classification

Cell Biology
Medical Bioscience
Pathobiology

Publication Identifiers

DOI: https://doi.org/10.3390/ijms232213976

URI (permanent link to this page)

https://res.slu.se/id/publ/119904