Potato as a Model for Field Trials with Modified Gene Functions in Research and Translational Experiments

Sammanfattning

Gene technology and editing are not only biotechnological techniques for creating new crop varieties but are also tools for researchers to discover gene functions. Field trial following laboratory experiments is an important step in order to evaluate new functions since many phenotypes, and combinations thereof, are difficult to detect in controlled environments and molecular analyses are nowadays possible to do in the field. Here we describe a standard protocol for creating new potato lines and producing seed tubers for field trials within 1 year.

Nyckelord

Potato; Solanum tuberosum; Field trials; Agrobacterium transformation; Seed tuber production; Micro tuber; In vitro long-term storage

Publicerad i

Methods in Molecular Biology
2021, nummer: 2354, sidor: 111-122 Titel: Solanum tuberosum : Methods and Protocols
ISBN: 978-1-0716-1608-6, eISBN: 978-1-0716-1609-3Utgivare: Springer

SLU författare

• Phuong, Nam Kieu

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

• Lenman, Marit

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

• Andreasson, Erik

  • Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Växtbioteknologi


Publikationens identifierare

DOI: https://doi.org/10.1007/978-1-0716-1609-3_5

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/120095