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Forskningsartikel - Refereegranskat, 2023

Genome-Wide Characterization and Analysis of bHLH Transcription Factors Related to Anthocyanin Biosynthesis in Cinnamomum camphora ('Gantong 1')

Gong, Xue; Shen, Tengfei; Li, Xiuqi; Lin, Hanbin; Chen, Caihui; Li, Huihu; Wu, Zhaoxiang; Liu, Qiaoli; Xu, Meng; Zhang, Bo; Zhong, Yongda


Cinnamomum camphora is one of the most commonly used tree species in landscaping. Improving its ornamental traits, particularly bark and leaf colors, is one of the key breeding goals. The basic helix-loop-helix (bHLH) transcription factors (TFs) are crucial in controlling anthocyanin biosynthesis in many plants. However, their role in C. camphora remains largely unknown. In this study, we identified 150 bHLH TFs (CcbHLHs) using natural mutant C. camphora 'Gantong 1', which has unusual bark and leaf colors. Phylogenetic analysis revealed that 150 CcbHLHs were divided into 26 subfamilies which shared similar gene structures and conserved motifs. According to the protein homology analysis, we identified four candidate CcbHLHs that were highly conserved compared to the TT8 protein in A. thaliana. These TFs are potentially involved in anthocyanin biosynthesis in C. camphora. RNA-seq analysis revealed specific expression patterns of CcbHLHs in different tissue types. Furthermore, we verified expression patterns of seven CcbHLHs (CcbHLH001, CcbHLH015, CcbHLH017, CcbHLH022, CcbHLH101, CcbHLH118, and CcbHLH134) in various tissue types at different growth stages using qRT-PCR. This study opens a new avenue for subsequent research on anthocyanin biosynthesis regulated by CcbHLH TFs in C. camphora.


anthocyanin; Cinnamomum camphora; basic helix-loop-helix (bHLH) transcription factors; collinearity analysis; RNA sequencing

Publicerad i

International Journal of Molecular Sciences
2023, Volym: 24, nummer: 4, artikelnummer: 3498
Utgivare: MDPI

    UKÄ forskningsämne

    Forest Science
    Biochemistry and Molecular Biology

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