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Forskningsartikel2023Vetenskapligt granskadÖppen tillgång

Liquid-Liquid Phase Separation Primes Spider Silk Proteins for Fiber Formation via a Conditional Sticker Domain

Leppert, Axel; Chen, Gefei; Lama, Dilraj; Sahin, Cagla; Railaite, Vaida; Shilkova, Olga; Arndt, Tina; Marklund, Erik G.; Lane, David P.; Rising, Anna; Landreh, Michael

Sammanfattning

Many protein condensates can convert to fibrillar aggregates, but the underlying mechanisms are unclear. Liquid-liquid phase separation (LLPS) of spider silk proteins, spidroins, suggests a regulatory switch between both states. Here, we combine microscopy and native mass spectrometry to investigate the influence of protein sequence, ions, and regulatory domains on spidroin LLPS. We find that salting out-effects drive LLPS via low-affinity stickers in the repeat domains. Interestingly, conditions that enable LLPS simultaneously cause dissociation of the dimeric C-terminal domain (CTD), priming it for aggregation. Since the CTD enhances LLPS of spidroins but is also required for their conversion into amyloid-like fibers, we expand the stickers and spacers-model of phase separation with the concept of folded domains as conditional stickers that represent regulatory units.

Nyckelord

Phase separation; native mass spectrometry; stickers and spacers-model; functional amyloid

Publicerad i

Nano Letters
2023, Volym: 23, nummer: 12, sidor: 5836-5841 Utgivare: AMER CHEMICAL SOC

    UKÄ forskningsämne

    Biokemi och molekylärbiologi

    Publikationens identifierare

    DOI: https://doi.org/10.1021/acs.nanolett.3c00773

    Permanent länk till denna sida (URI)

    https://res.slu.se/id/publ/122255