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Research article - Peer-reviewed, 2023

High fructan barley lines produced by selective breeding may alter beta-glucan and amylopectin molecular structure

Jayarathna, Shishanthi; Jin, Yunkai; Dotsenko, Gleb; Fei, Mingliang; Andersson, Mariette; Andersson, Annica A. M.; Sun, Chuanxin; Andersson, Roger

Abstract

Six cross-bred barley lines developed by a breeding strategy with the target to enhance the fructan synthesis activity and reduce the fructan hydrolysis activity were analyzed together with their parental lines, and a reference line (Gustav) to determine whether the breeding strategy also affected the content and molecular structure of amylopectin and beta-glucan. The highest fructan and beta-glucan content achieved in the novel barley lines was 8.6 % and 12 %, respectively (12.3-fold and 3.2-fold higher than in Gustav). The lines with low fructan synthesis activity had higher starch content, smaller building blocks in amylopectin, and smaller structural units of beta-glucans than the lines with high-fructan synthesis activity. Correlation analysis confirmed that low starch content was associated with high amylose, fructan, and beta-glucan content, and larger building blocks in amylopectin.

Keywords

Fructan; Starch; beta-Glucan; Amylopectin; Building block structure

Published in

Carbohydrate Polymers
2023, Volume: 316, article number: 121030
Publisher: ELSEVIER SCI LTD