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Forskningsartikel2020Vetenskapligt granskadÖppen tillgång

Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania

Maganira, J.D.; Kidima, W.; Mwita, C.J.; Halvarsson, P.; Höglund, J.


The presence ofTaenia solium DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pig-keeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I (cox1) gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial cox1 gene of T. solium. A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas T. solium DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of T. solium DNA in this study spells out a low risk of exposure to T. solium eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening T. solium eggs in soil.


ddPCR; eggs; Kongwa; soil contamination; Taenia solium; Tanzania

Publicerad i

Infection Ecology & Epidemiology
2020, Volym: 10, nummer: 1, artikelnummer: 1772668
Utgivare: Taylor and Francis Ltd.