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Bokkapitel2020Vetenskapligt granskad

Challenges of small RNA technology

Singh, R.K.; Krishnamachari, A.; Sharaff, M.

Sammanfattning

Plant small RNAs are largely non-coding, regulatory RNAs that act with a sequence-specific binding capacity to regulate target gene expression, either before transcription, a process called transcriptional gene silencing (TGS), or after transcription, a process called post-transcriptional gene silencing (PTGS) or RNA interference (RNAi), in general. Emerging evidence from high-throughput sequencing technologies leads us in characterizing several categories and groups of small RNAs. They play essential roles in complex biological processes under various physiological conditions. Small RNA-mediated gene silencing has been proven as a promising and powerful genetic tool for the improvement of crop plants and agriculture. It has emerged as a means to probe the function of a gene of interest on a whole-genome scale. Parallel to the enormous potential and applications, this technology has met with several new challenges, such as the off-target effects, delivery of optimum and sufficient small RNAs at the targeted cells, its measure for persistence in the environment, and several ethical issues.

Nyckelord

Next-generation sequencing; Non-coding RNAs; Off-target effects; Post-transcriptional gene silencing; RNA interference; Small RNA technology; Small RNAs; Spray-induced gene silencing

Publicerad i

Titel: Plant Small RNA : Biogenesis, Regulation and Application
ISBN: 9780128171127, eISBN: 9780128173367Utgivare: Elsevier

    UKÄ forskningsämne

    Genetik

    Publikationens identifierare

    DOI: https://doi.org/10.1016/B978-0-12-817112-7.00024-9

    Permanent länk till denna sida (URI)

    https://res.slu.se/id/publ/129836