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Book chapter2013Peer reviewed

Identification and verification of microRNAs by high-throughput sequencing

Hällman, J.; Avesson, L.; Reimegård, J.; Käller, M.; Söderbom, F.

Abstract

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs. © Springer Science+Business Media, LLC 2013.

Keywords

Bioinformatics; High-throughput sequencing; MicroRNA; Northern blot; Small RNA

Published in

Methods in Molecular Biology
2013, volume: 983, number: 983, pages: 125-138
Title: Dictyostelium discoideum Protocols
Publisher: Humana Press Inc.

SLU Authors

  • Avesson, Lotta

    • Department of Molecular Biology, Swedish University of Agricultural Sciences
    • Garvan Institute of Medical Research

  • Söderbom, Fredrik

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

UKÄ Subject classification

Bioinformatics (Computational Biology)
Biochemistry and Molecular Biology

Publication identifier

  • DOI: https://doi.org/10.1007/978-1-62703-302-2_7
  • ISBN: 9781627033015
  • eISBN: 978-1-62703-302-2

Permanent link to this page (URI)

https://res.slu.se/id/publ/132195