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Forskningsartikel - Refereegranskat, 2003

A genetic linkage map of a tetraploid Salix viminalis x S. dasyclados hybrid based on AFLP markers

Rönnberg-Wästljung AC, Tsarouhas V, Semerikov V, Lagercrantz U

Sammanfattning

In order to study the inheritance of frost resistance as well as growth traits genetic maps specific for two tetraploid Salix dasyclados x S. viminalis hybrids based on a three-generation pedigree have been constructed. The F1 generation was created by an interspecific cross between a high producing, commercial diploid S. viminalis clone (Jorunn) and a natural, frost resistant, hexaploid S. dasyclados clone (SW901290). Single dose AFLP markers segregating in 1:1, 3:1 or 1:2:1 ratios in 92 F2 individuals were used. In the map of the male F1 parent 269 markers were distributed over 37 major linkage groups and thirty-one minor groups covering 1820 cM of the genome. The map of the female F1 parent consists of 248 markers assigned to 35 major linkage groups and twenty-two minor groups covering 1640 cM of the genome. For eight linkage groups, integration between male and female groups was possible due to 3:1 markers that linked both to male and female markers segregating in a 1:1 ratio. Seventy-four percent of the markers originate from the male grandparent (SW901290), which could be expected due to the hexaploid level in the S. dasyclados grandparent. In average eleven percent of the markers on the maps showed a distorted segregation

Nyckelord

genetic linkage map; single-dose markers; tetraploidy; AFLP-markers; Salix viminalis; Salix dasyclados

Publicerad i

Forest Genetics
2003, Volym: 10, nummer: 3, sidor: 185-194