Toxin production by and adhesive properties of Clostridium difficile isolated from humans and horses with antibiotic-associated diarrhea

Sammanfattning

Clostridium difficile is a common nosocomial pathogen in humans and animals that causes diarrhea and colitis following antibiotic therapy. Isolates of C. difficile obtained from faecal material from 20 human patients and 6 equine subjects with antibiotic-associated diarrhea were investigated regarding production of toxins A and B, their capacity to adhere to the human intestinal Caco-2 cell line and equine intestinal cells, and for the presence of fimbriae. The results showed that most (17/20) of the human clinical isolates produced both toxins A and B. One of the human isolates proved toxin A-negative/toxin B-positive. All (6/6) horse isolates were positive for both toxins A and B. Both the human and horse isolates possessed the capacity to adhere, to varying degree, to human and equine intestinal cells. It appeared that human isolates produced greater amounts of toxin B, and that there was a host-species dependency on ability to attach to intestinal epithelial cells. No fimbriae were found in any of the investigated isolates. (c) 2007 Elsevier Ltd. All rights reserved

Publicerad i

Comparative Immunology, Microbiology and Infectious Diseases
2007, volym: 30, nummer: 3, sidor: 163-174
Utgivare: ELSEVIER SCI LTD

SLU författare

• Krovacek, Karel

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Husdjursvetenskap
Veterinärmedicin


Publikationens identifierare

DOI: https://doi.org/10.1016/j.cimid.2006.11.006

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/14709