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Forskningsartikel2007Vetenskapligt granskadÖppen tillgång

Comparison of fertility results after vaginal insemination using different thawing procedures and packages for frozen ram semen

Paulenz H, Adnoy T, Soderquist L


Background: The effect of different thawing procedures for ram semen frozen in minitubes and mini straws on the fertility of sheep was tested in a field trial. Methods: Altogether, 719 Norwegian Crossbred ewes, aged between six months and six-and-a-half years from 8 farms, were inseminated vaginally in natural oestrus with frozen- thawed semen. Minitubes were thawed at 70 degrees C for 8 sec (T70) and mini straws either at 50 degrees C for 9 sec (S50) or at 35 degrees C for 12 sec (S35). Results: Vaginal insemination with 200 x 10(6) spermatozoa resulted in 25-days non-return rates of 63.2, 59.6, and 62.5% (overall 61.8%), respectively, and lambing rates of 56.8, 55.0, and 59.2% (overall 57.0%), respectively. No significant effect on fertility (as 25-days non-return-or lambing rate) was seen for straw type/thawing temperature (P = 0.5/0.5), but semen filled in mini straws and thawed at 35 degrees C resulted numerically in the highest lambing rate ( 59.2%). A significant effect was, however, seen for farmer (P = > 0.0001/> 0.0001) and ram (P = 0.009/0.002). Moreover, age of the ewes had a significant effect on the NR rate (0.007), but not on lambing rate (P = 0.2). Conclusion: A vaginal deposition of frozen ram semen containing approximately 200 x 10(6) spermatozoa, filled in mini straws and thawed at 35 degrees C is a simplified technique that under field conditions and used on a do-it-yourself regime gives acceptable lambing rates in Norway

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Acta Veterinaria Scandinavica
2007, Volym: 49, artikelnummer: 26Utgivare: BIOMED CENTRAL LTD

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