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Research article - Peer-reviewed, 2003

Two potato proteins, including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro

Guo DY, Spetz C, Saarma M, Valkonen JPT

Abstract

Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions

Published in

Molecular plant-microbe interactions
2003, volume: 16, number: 5, pages: 405-410
Publisher: AMER PHYTOPATHOLOGICAL SOC

Authors' information

Spetz, Carl
Swedish University of Agricultural Sciences, Department of Plant Biology
Valkonen, Jari
Swedish University of Agricultural Sciences, Department of Plant Biology
Guo, D
Saarma, M

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1094/MPMI.2003.16.5.405

URI (permanent link to this page)

https://res.slu.se/id/publ/1894