Spetz, Carl
- Department of Plant Biology, Swedish University of Agricultural Sciences
Abstract
Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.
Published in
Molecular plant-microbe interactions
2003, volume: 16, number: 5, pages: 405-410
Publisher: AMER PHYTOPATHOLOGICAL SOC
SLU Authors
Valkonen, Jari
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1094/MPMI.2003.16.5.405
Permanent link to this page (URI)
https://res.slu.se/id/publ/1894