Comparison of T-RFLP and DGGE techniques to assess denitrifier community composition in soil

Abstract

Terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE) and subsequent statistical analysis were compared with assess denitrifier community composition in agricultural soil based on the nosZ gene, encoding the nitrous oxide reductase. Analysis of binary or relative abundance-based metric and semi-metric distance matrices provided similar results for DGGE, but not for T-RFLP. Moreover, DGGE had a higher resolution than T-RFLP and binary data was better for discriminating between samples.

Keywords

agriculture; community fingerprint techniques; denitrification; microbial ecology; soil diversity

Published in

Letters in Applied Microbiology
2009, volume: 48, number: 1, pages: 145-148

SLU Authors

• Enwall, Karin

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Hallin, Sara

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science
Environmental Sciences and Nature Conservation

Publication identifier

• DOI: https://doi.org/10.1111/j.1472-765X.2008.02498.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/20521