Skip to main content
SLU publication database (SLUpub)

Abstract

The objective of this work was to find regressions between minor milk proteins or protein fragments in the casein or sweet whey fraction and cheese yield because the effect of major milk proteins was evaluated in a previous study. Proteomic methods involving 2-dimensional gel electrophoresis and mass spectrometry in combination with multivariate data analysis were used to study the effect of variations in milk protein composition in chymosin separated whey and casein fractions on cheese yield. By mass spectrometry, a range of proteins significant for the cheese yield was identified. Among others, a C-terminal fragment of beta-casein had a positive effect on the cheese yield expressed as grams of cheese per 100 g of milk, whereas several other minor fragments of beta-, alpha(s1)-, and alpha(s2)- casein had positive effects on the transfer of protein from milk to cheese. However, the individual effect of each identified protein was relatively low. Therefore, further studies of the relations between different proteins/peptides in the rennet casein or sweet whey fractions and cheese yield are needed for advanced understanding and prediction of cheese yield.

Keywords

rennet casein; sweet whey protein; proteomics; protein transition number

Published in

Journal of Dairy Science
2008, volume: 91, number: 10, pages: 3787-3797
Publisher: AMER DAIRY SCIENCE ASSOC

SLU Authors

UKÄ Subject classification

Animal and Dairy Science
Food Science
Veterinary Science

Publication identifier

  • DOI: https://doi.org/10.3168/jds.2008-1022

Permanent link to this page (URI)

https://res.slu.se/id/publ/20656