Sammanfattning

Floral organ development in plants is apart from nuclear control also influenced by nuclear-mitochondrial interactions. This is demonstrated by cytoplasmic male sterility (CMS), which is manifested as an abnormal development of stamens and pollen. In addition to the inhibited pollen production, homeotic-like conversions of the anthers into for instance carpel like structures with ovules and stigmas are observed. Such conversions are found in CMS-lines derived from somatic hybrids between Brassica napus and Arabidopsis thaliana. The aberrations can be restored by nuclear genes confirming the interactions of nuclear and mitochondrial genes. CMS-plants are female fertile and will produce normal seeds if crossed with male fertile plants. This could be used in plant breeding for production of hybrid seeds. The long-term goal of my PhD-project is to investigate the nuclear-mitochondrial interactions and expression patterns during development of fertile plants as well as CMS and restored alloplasmic lines. This will be done by comparing the gene expression in different plant tissues and organs. In order to detect the expression patterns that are unique for the different morphological aberrations the plant material will be cultivated under controlled conditions and followed in detail regarding differentiation and development. By comparative RNA/DNA analysis on microarray slides to which the complete or part of the genome of A. thaliana has been spotted a screening of RNA isolated from the different alloplasmic lines will be preformed. In this poster a characterisation of the material to be investigated will be made as well as the microarray method to be used. The plant material consists of CMS B. napus (+) A. thaliana lines that have been continuously backcrossed with pollen from fertile B. napus. Partial restoration of homeotic conversions and pollen production has been obtained in some lines by introduction of A. thaliana nuclear DNA. A general discussion about microarrays, techniques how to prepare RNA from very small amounts of tissue, RNA amplification and cDNA preparation will be covered. Finally results from the staining procedure and hybridisation to microarray slides will be presented

Publicerad i

Konferens

Current Challenges in Plant Biology, Second SPPS PhD Student Conference, Åbo

SLU författare

• Carlsson, Jenny

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Glimelius, Kristina

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Jordbruksvetenskap


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https://res.slu.se/id/publ/23111