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Conference abstract2008

Effects of sesamin on lipid metabolism in salmon hepatocytes

Trattner, Sofia; Østbye, Tone Kari; Ruyter, Bente; Kamal-Eldin, Afaf; Pickova, Jana

Abstract

In previous studies sesamin has been shown to have impact on fatty acid and vitamin E metabolism in salmonids. The most significant change in fatty acid composition was the increased amount of 22:6n-3 (DHA) with up to 37% in white muscle. In a in vitro study on salmon (Salmo salar L) hepatocytes, incubation with C14 18:3n-3 and sesamin, we could demonstrate that sesamin addition increased elongation and desaturation of 18:3n-3 to DHA. The amount of DHA in the sesamin group was 20.2±2.05% compared to 10.5±0.10% in the control group . Also the total amount of LCn-3-PUFAs were higher in the sesamin group (p = 0.01). Addition of sesamin, increased also the total capacity in hepatocytes (p = 0.006). The major β-oxidation product formed from 18:3n-3 were acetate, constituting 53.5±5.4% of the acid soluble oxidation products (ASP) in the control group and 72.6±1.7%, respectively in the sesamin group (P < 0.001). The expression of ctp1 was significantly up regulated by a factor 3.8 (p = 0.001), and expression of PPARγ was down regulated by a factor 2.2 (p = 0.001). This effects on gene expression could indicate an increased β-oxidation and a decreased synthesis of lipids

Conference

Eour Fed Lipids