Unell, Maria
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Research article2009Peer reviewedOpen access
Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome
Unell, Maria; Abraham, Paul E.; Shah, Manesh; Zhang, Bing; Rueckert, Christian; VerBerkmoes, Nathan C.; Jansson, Janet K.
Abstract
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
Keywords
Arthrobacter chlorophenolicus; 4-chlorophenol; 4-nitrophenol; phenol; shotgun proteomics; 2D-LC-ES-MS/MS; hydroxyquinol dioxygenase
Published in
Journal of Proteome Research
2009, Volume: 8, number: 4, pages: 1953-1964
Jansson, Janet
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Lawrence Berkeley National Laboratory (Berkeley Lab)
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Environmental Sciences related to Agriculture and Land-use
Publication identifier
DOI: https://doi.org/10.1021/pr800897c
Permanent link to this page (URI)
https://res.slu.se/id/publ/26199