Unell, Maria
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
Keywords
Arthrobacter chlorophenolicus; 4-chlorophenol; 4-nitrophenol; phenol; shotgun proteomics; 2D-LC-ES-MS/MS; hydroxyquinol dioxygenase
Published in
Journal of Proteome Research
2009, volume: 8, number: 4, pages: 1953-1964
SLU Authors
Jansson, Janet
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Lawrence Berkeley National Laboratory (Berkeley Lab)
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Environmental Sciences and Nature Conservation
Publication identifier
- DOI: https://doi.org/10.1021/pr800897c
Permanent link to this page (URI)
https://res.slu.se/id/publ/26199