Selection of boar spermatozoa using centrifugation on a glycidoxypropyltrimethoxysilane-coated silica colloid

Abstract

Use of sperm separation methods such as density gradient centrifugation for selecting the best spermatozoa for animal breeding is constrained by the problem of dealing with the large volumes of ejaculate produced by the males of some species, such as boars. The purpose Of this Study was to compare density gradient centrifugation (DGC) with centrifugation on a single layer of colloid (SLC) for the preparation of ejaculated boar spermatozoa using Androcoll (TM)-P. There was no difference between the two techniques in terms of sperm motility or duration of motility after selection, and sperm motility was retained for at least 24 h longer in the centrifuged sperm preparations than in controls (uncentrifuged aliquots). Sperm motility was significantly better (P

Keywords

Boar spermatozoa; Colloids; Density gradient centrifugation; Single layer centrifugation

Published in

Journal of Reproduction and Development
2009, volume: 55, number: 5, pages: 547-552

SLU Authors

• Saravia-Ramos, Fernando

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• van Wienen, Marjet

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Wallgren, Margareta

  • Quality Genetics

• Rodriguez, Heriberto

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science

Publication identifier

• DOI: https://doi.org/10.1262/jrd.20243

Permanent link to this page (URI)

https://res.slu.se/id/publ/27682