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Forskningsartikel2004Vetenskapligt granskad

Enniatins of Fusarium sp. Strain F31 and Their Inhibition of Botrytis cinerea Spore Germination

Capieau, K; Stenlid, J; Stenström, E; Pohanka, Anton; Broberg, Anders; Kenne, Lennart


A spectrum of enniatins was isolated from Fusarium sp. strain F31 by bioassay-guided isolation directed against Botrytis cinerea. Two new enniatins, J2 (7) and J3 (8), were co-isolated and both contained, in addition to three hydroxyisovaleric acid units, N-methylated l-alanine, l-valine, and l-isoleucine units, differing only in their primary sequence. Two other enniatins, named enniatin J1 (1) and enniatin K1 (6), each containing two N-Me-l-Val units and one N-Me-l-Ala or α-N-Me-l-butyric acid unit, respectively, were isolated for the first time without directed biosynthesis. The enniatin structures were elucidated by spectroscopic and chemical methods, and the absolute configuration of the amino acids (l) and hydroxyisovaleric acid (d) was consistent with all previously isolated enniatins. The known enniatins B (2), B1 (4), B2 (5), and B4 (3) were also isolated. The minimum inhibitory concentration of pure enniatins against Botrytis cinerea was 75 μg/mL.

Publicerad i

Journal of Natural Products
2004, Volym: 67, nummer: 5, sidor: 851-857
Utgivare: American Chemical Society